Behavior of Hemoglobin Fractions in healthy adults by HPLC
DOI:
https://doi.org/10.5377/rtu.v11i31.14290Keywords:
hemoglobin fractions, hemoglobin A1,A2, HPLC, holding timeAbstract
The blood is composed of hemoglobin that functionally allows cellular respiration in turn the structure of hemoglobin is highly specialized and its measurement is decisive to know the state of health of the individual beyond routine analyzes. Normally hemoglobin electrophoresis at alkaline pH is used to determine fractions however HPLC is worldwide accepted as the confirmatory method. (Ondei, Zamaro, Mangonaro, Valéncio, & Bonini-Domingos, 2007) In this research, the fractions of hemoglobin were quantified by HPLC using the D-10 equipment of BioRad to 158 apparently healthy subjects, using exclusion criteria and leaving 72 on which the percentages of hemoglobins were analyzed, the results were 96.03% for Hb A1, 2.92% for Fraction A2, 0.73% of Fetal Hb and 5.32% for Hb A1c. These results are similar to those made in other countries. Regarding hemoglobin A2 there was a decrease according to age and in women between 30 to 40 years showed the highest averages 1.4% while the male of 41-51 years reported lower values than women 0.76%. The HPLC method turned out to be a very stable procedure of rapid realization and allows the classification in fractions of Hb obtaining subfractions and variants of normal and abnormal hemoglobin. This research allows us to stop the neonatal screening tests carried out in Nicaragua and is the basis for the continuation of research on hemoglobins.
Downloads
References
Azevedo A, S. P., & V, T. (2010). Valores de referencia para hemograma en una poblaciónmetropolitana de Lisboa. Acta Medica Portuguesa , 597-604.
B.Amor, H, M., B, H., I, G., & R. (2012). Valores de referencia en 1000 adultos. Pasteur Tunis , 47-61.
Benito-Godino, A. S., Vergara Chozas, J. M., Marquez Ronchel, A., Barrero-Alor, F., Papay Ramirez, L., Coronado Alvarez, N. M., y otros. (2014). Multicentre evaluation og glycated haemoglobin (HbA1c) of Roche Diagnostics in Andalucia. Clinical Biochemistry, 4;4C.
BIO_RAD. (23 de septiembre de 2010). Manual de operaciones BIORAD D10 Quimica Clinica. Recuperado el 23 de agosto de 2020, de http://mdkhospital.com/documents/Hemoglobin%20A1c%20D10.pdf
Ciesla, B. (2007). Hematology in practice . Philadelphia: F.A.Davis Company /www.fadavis.com.
Figuereido, M. S. (2015). The importance of hemoglobin A2 determination . revista Brasileira de hematologia e hemoterapia , 287-9.
Greer, J. P., Arber, D. A., & Gladel, B. (2014). Wintrobe's Clinical Hematology. Philadelphia: Lippincott.
Hamid, G. A. (2013). Clinical hematology. Yemen: ADEN UNIVERSITY.
IFCC Federación Internacional de Quimica Clinica. (2016). IFCC. SEQC .
J.M.Vergara, & A, S.-b. (s.f.). Comparación de los resultados de hemoglobina glicada por dos sistemas de cromatografia liquida HPLC. Journal Congreso España .
Joutovsky, M. (2004). HPLC Retention Time as a Diagnostic Tool for Hemoglobin Variants and Hemoglobinopathies: A Study of 60000 Samples in a Clinical Diagnostic Laboratory. AACC's Clinical Chemistry. Vol 50 (10). https://doi.org/10.1373/clinchem.2004.034991
Kordys, M. E., Gallego, F., & Collino, C. (2014). Evaluación de métodos y establecimiento de Valores de Referencia hematológicos para la población del Gran Mendoza ". Revista de la asociación Bioquímica Argentina , Vol.78 No2.
Ondei, L., Zamaro, P. J., Mangonaro, P. H., Valéncio, C. R., & Bonini-Domingos, C. R. (2007). HPLC determination of hemoglobins to establish reference values with the aid of statistics and informatics. Genetics and Molecular Research , 453-460.
Ramos, N. (2016). CATLAB informa. Hematología CATLAB,Butlleti No 66-Mes Gener, 1-11.
Rodríguez Romero, W. E., Villalobos Fernández, J., Salas Abarca, P., Hong Yuan, L., & Chui, D. H. (2012). Hemoglobina Raleigh en Costa Rica detectada omo un valor falsamente elevado de hemoglobina glicosilada. Rev Biomed , 23:33-38.
Soler Pont, M., & Timon, M. (2014). Que pasa cuando el HbA1c miente por defecto . Butlletí, vol 32 , 3.
Nogueira dos Santos, Cavalcante Barbosa, dos Santos, Silva Diniz, Lemos, & Pinheiro Goncalves (2011). Triagem para hemoglobinas variantes em população adulta no Estado do Ceará Frequency of variant hemoglobins in the adult populations of State of Ceará Universidade Federal do Ceará. Correspondência: Thayna Nogueira dos Santos. Rua Paraná, 700. Apt. 102 – Demócrito Rocha. Fortaleza-CE. Tel.: (55-85) 88646683.
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 National Autonomous University of Nicaragua, Managua (UNAN-Managua).
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
The authors who publish in this journal agree to the following terms.
- The author or authors of the articles, essays or research grant the National Autonomous University of Nicaragua, Managua (UNAN-Managua) the editing rights (copyright) of the submitted work, therefore the University has the exclusive right to publish the article for the entire copyright period.
- These copyrights/authors authorize Torreón Universitario Magazine and the University to edit and disseminate/publish the article in said Magazine, including printed and electronic reproduction, storage, retrieval and any other type of publication, and sources of secondary information as services. of summaries and databases, they also empower it to protect the article against unauthorized use for dissemination by printed or electronic media (PDF, HTML, EPUB, XML or others).
License for use of content
The magazine uses the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License.
Under this statement:
This journal is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. It can be copied, distributed and transmitted publicly as long as the author and source are cited (Revista Torreón Universitario), it should not be modified or used for any commercial purpose. The full license can be found at http://creativecommons.org/licenses/by-nc-nd/4.0/.
