Behavior of Hemoglobin Fractions in healthy adults by HPLC

Authors

DOI:

https://doi.org/10.5377/rtu.v11i31.14290

Keywords:

hemoglobin fractions, hemoglobin A1,A2, HPLC, holding time

Abstract

The blood is composed of hemoglobin that functionally allows cellular respiration in turn the structure of hemoglobin is highly specialized and its measurement is decisive to know the state of health of the individual beyond routine analyzes. Normally hemoglobin electrophoresis at alkaline pH is used to determine fractions however HPLC is worldwide accepted as the confirmatory method. (Ondei, Zamaro, Mangonaro, Valéncio, & Bonini-Domingos, 2007) In this research, the fractions of hemoglobin were quantified by HPLC using the D-10 equipment of BioRad to 158 apparently healthy subjects, using exclusion criteria and leaving 72 on which the percentages of hemoglobins were analyzed, the results were 96.03% for Hb A1, 2.92% for Fraction A2, 0.73% of Fetal Hb and 5.32% for Hb A1c. These results are similar to those made in other countries. Regarding hemoglobin A2 there was a decrease according to age and in women between 30 to 40 years showed the highest averages 1.4% while the male of 41-51 years reported lower values than women 0.76%. The HPLC method turned out to be a very stable procedure of rapid realization and allows the classification in fractions of Hb obtaining subfractions and variants of normal and abnormal hemoglobin. This research allows us to stop the neonatal screening tests carried out in Nicaragua and is the basis for the continuation of research on hemoglobins.

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Published

31-05-2022

How to Cite

Ortega Valdés, L. L., Vanegas López , J., & Ruiz Saldívar, D. M. (2022). Behavior of Hemoglobin Fractions in healthy adults by HPLC. Revista Torreón Universitario, 11(31), 92–104. https://doi.org/10.5377/rtu.v11i31.14290

Issue

Section

Health and Social services

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