Viability of spores of Aspergillus awamori immobilized in cryogels
DOI:
https://doi.org/10.5377/rtu.v10i29.12739Keywords:
Immobilization by entrapment, polivinyl alcoholAbstract
The work consisted of immobilizing Aspergillus awamori spores in polyvinil alcohol (PVA) at different concentrations 10, 12 y 15% (w/v). The viability of the immobilized spores was compared to the free spores, by the conventional plate counting method. The imobilization technique was by entrapment, it consisted of a suspension of spores in the polymer and then it was frozen at -18 ºC for 24 hours. Viability was similar in immobilized and frees pores, this indicates that there are probably no toxic effects of the polymer and that exposure to low temperatures does not damage the structure of the spores. Spores leakage was different in each case of immobilization at 24 hours, the least leakage was with spores at 15% and the highest at 12%. At 96 hours, the lowest was with spores immobilized at 10%, followed by 15% and the highest spores immobilized at 12%, thus remaining until 120 hours.
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