Bacterial bioprospecting in sediment from the Chiquito River, in León, Nicaragua during dry and rainy seasons.
DOI:
https://doi.org/10.5377/rtu.v11i32.15034Keywords:
Bioprospecting, heavy metals, sedimentsAbstract
Two sample collection (dry and rainy season) were carried out in sediments exposed to tannery effluents and domestic wastewater (Exposed Sample); and in sediment visually contaminated by domestic wastewater (Reference Sample), with the aim of isolating and identifying bacteria according to Gram (structural) and concentrations of heavy metals of interest. The highest percentage of colonies found was in the exposed sediment, in the rainy season (66.67), identified as Gram- and the lowest was 33.33 in the same season, corresponding to Gram+. The highest enzymatic activity of lipase was in the MR in summer (3219 µg of Pnpg/10 min) and the lowest activity was obtained in the ME in winter (1710.6 µg of Pnp/g.10 min). The variation of aqueous pH and moisture content was not significant at both sites, both in the dry and rainy seasons. Hexavalent Chromium and Arsenic were not detected during the sampling periods. The texture of the sediment is sandy, which probably facilitates the transport of heavy metals. Iron concentration was similar in summer and winter, in the MR and ME (36.416 and 36.428 g.Kg -1 respectively). Lead concentrations were 12, 12,242 and 22 mg.kg-1, at the MR winter, MR summer and ME summer points, respectively; in the ME in winter it was not detected. The results suggest that, due to microbial interaction with metals, they have created resistance and tolerance through different mechanisms. It is necessary to know the minimum inhibitory concentrations of the isolated bacteria and to enhance their application in Bioremediation.
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