Evaluation of DNA extraction methodologies from recalcitrant plants

Authors

  • Taryn Suazo Ubieta National Autonomous University of Nicaragua, Managua. Biotechnology Laboratory
  • Samantha Miranda Calero National Autonomous University of Nicaragua, Managua. Biotechnology Laboratory
  • Ena Rivers Carcache National Autonomous University of Nicaragua, Managua. Biotechnology Laboratory
  • Martha Lacayo Romero National Autonomous University of Nicaragua, Managua. Biotechnology Laboratory
  • Daniel Tenorio Juárez National Autonomous University of Nicaragua, Managua. Biotechnology Laboratory

DOI:

https://doi.org/10.5377/torreon.v9i24.9723

Keywords:

DNA, extraction, commercial kits, protocols, recalcitrant

Abstract

Obtention of high-quality purified deoxyribonucleic acid (DNA) is key for the subsequent application of molecular techniques during the characterization of species under study. In this regard, plant extracts can be challenging due to its complex metabolites composition and to the fact that often these chemicals concentration differs across varieties and even samples. This is, for instance, the case for recalcitrant samples of cocoa varieties (Theobroma cacao) and coffee (Coffea arabica), where DNA loss can occur during sample maceration due to phenolization. In this research, three commercial kits, already known by producing excellent recoveries for non-recalcitrant varieties, were evaluated. Additionally, the modification of traditional protocols [Doyle & Doyle (1987) and Doyle & Doyle (1990)] was also evaluated. Results obtained confirm the effect of variety and species on DNA extraction and for some species modified methods may yield DNA recovery and purity higher than those obtained by commercial kits.

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Published

07-05-2020

How to Cite

Suazo Ubieta, T., Miranda Calero, S., Rivers Carcache, E., Lacayo Romero, M., & Tenorio Juárez, D. (2020). Evaluation of DNA extraction methodologies from recalcitrant plants. Revista Torreón Universitario, 9(24), 45–57. https://doi.org/10.5377/torreon.v9i24.9723

Issue

Section

SCIENTIFIC ARTICLES

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